Multicolor Flow Cytometry Panel Design

Panel design and multicolor flow cytometry information advances in both flow cytometry reagents and instrumentation allow researchers to run increasingly complex multicolor experiments.

Multicolor flow cytometry panel design.

Flow cytometry multicolour panel design well planned and executed assays can allow the detection and monitoring of multiple parameters simultaneously in complex samples. However increasing the number of antigens and fluorochromes also increases the complexity of the experimental design. Know the configuration of the instrument being used laser and filters before you begin. Constructing multicolor panels for flow cytometry can be a challenging task.

Use a tool like the molecular probes fluorescence spectraviewer to visualize the spectral overlap of fluorophores. Keep adding until you have all the markers you want. Such multicolour assays can produce an enormous amount of data from precious or limited samples. To find more information check your cytometer manual view our instrument guide page or check with your local flow core facility.

Flow cytometry is a powerful tool for identifying and analyzing multiple antigens simultaneously. Empower your multicolor panel design with bd tools and information assembled here to help you take full advantage of the power of multicolor flow cytometry. On this page you will find helpful tools and resources to support multicolor panel design workflow and experimental analysis. When designing a multicolor flow cytometry panel there are several key points to consider.

Follow these guidelines and recommendations to achieve optimized panels. Our comprehensive portfolio of conjugated antibodies is designed to help characterize cells through surface intracellular or secreted markers. Panel design education at bd we provide innovative instruments reagents and software to support scientists throughout the full continuum of flow cytometry experiments.